Presented By:
Jennifer Sandoval, M.S.
Speaker Biography:
Jennifer Sandoval signed up with Thermo Fisher Scientific in 2010 as a Technical Scientist and has actually supported genome modifying and analysis throughout her profession for over 17 years. A knowledgeable researcher with a background in pharmaceutical drug advancement, Jennifer’s specialized has actually been concentrated on the impacts of endothelin villains on hepatic hemodynamics in cirrhotic rats, along with genomic profiling analysis of skin development aspects and prostaglandin dehydrogenase markers in ovarian cancer. She holds a master’s degree in Biochemistry and Molecular Biology from the University of Arkansas Medical School in Little Rock, Arkansas. Jennifer is enthusiastic about brand-new innovations for genomic analysis and is constantly looking for methods to share brand-new findings with the clinical neighborhood.
Webinar:
Breaking through RT-qPCR limits in gene expression analysis
Webinar Abstract:
Variation in gene expression amongst cell types, tissues, and organisms is typically analyzed by reverse transcription quantitative PCR, or RT-qPCR. In this procedure, RNA is separated from samples of interest and reverse-transcribed into complementary DNA (cDNA). The levels of gene expression are then identified from the amount of cDNA enhanced by PCR. The preliminary phases of the RT-qPCR workflow, such as RNA seclusion and reverse transcription, can seriously affect the precision of the quantitation of gene expression.
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