SCOG Virtual Lecture Series - Christian Schmidl (Leibniz Institute for Immunotherapy, Regensburg)

‘ Integrated single-cell profiling dissects cell-state-specific enhancer landscapes of human tumor-infiltrating T cells’

Despite comprehensive research studies on the chromatin landscape of tired T cells, the transcriptional electrical wiring underlying the heterogeneous practical and inefficient states of human tumor-infiltrating lymphocytes (TILs) is incompletely comprehended. Here, we determine tissue-specific and basic gene-regulatory landscapes in the broad breadth of CD8+ TIL practical states covering 4 cancer entities utilizing single-cell chromatin profiling. We map enhancer-promoter interactions in human TILs by incorporating single-cell chromatin ease of access with single-cell RNA-seq information from growth entity-matching samples, and focus on crucial elements by super-enhancer analysis. Exposing entity-specific chromatin improvement in tired TILs, our analyses recognize a human core chromatin trajectory to TIL dysfunction and identify included crucial enhancers, transcriptional regulators, and decontrolled target genes in this procedure. We confirm enhancer policy at immunotherapeutically pertinent loci by targeting non-coding regulative aspects with powerful CRISPR activators and repressors. In summary, our research study offers a structure for understanding and controling cell-state-specific gene-regulatory hints from human growth penetrating lymphocytes.

Biography:
Christian Schmidl acquired his PhD in 2013 at the University of Regensburg under the guidance of Michael Rehli where he studied DNA methylation patterns in lymphocytes. After that he signed up with the Research Center for Molecular Medicine (CeMM) in Vienna as a postdoc in Christoph Bock’s laboratory, where he established and used chromatin mapping approaches to much better comprehend molecular policy of persistent lymphocytic leukaemia. In 2017, Christian seized the day to develops his own research study group at the Regensburg Center for Interventional Immunology (now Leibniz Institute for Immunotherapy), where he is studying gene-regulatory systems in T cells in the context of cancer.

More details:
https://www.singlecell.de; Twitter: @singlecellomics

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